| Filename | Total | Flagged As Poor Quality | Sequence Length | %GC |
|---|---|---|---|---|
| 1_FAD_5_2Aligned.sortedByCoord.out.bam | 24,871,513 | 0 | 23-76 | 45 |
| 1_FAD_5Aligned.sortedByCoord.out.bam | 23,538,193 | 0 | 24-76 | 45 |
| 10_FS_1_2Aligned.sortedByCoord.out.bam | 23,868,599 | 0 | 22-76 | 46 |
| 10_FS_1Aligned.sortedByCoord.out.bam | 22,556,636 | 0 | 23-76 | 46 |
| 11__-_5_2Aligned.sortedByCoord.out.bam | 23,202,507 | 0 | 23-76 | 45 |
| 11__-_5Aligned.sortedByCoord.out.bam | 21,951,190 | 0 | 23-76 | 45 |
| 12__-_4_2Aligned.sortedByCoord.out.bam | 24,120,299 | 0 | 23-76 | 45 |
| 12__-_4Aligned.sortedByCoord.out.bam | 22,807,843 | 0 | 23-76 | 45 |
| 13__-_3_2Aligned.sortedByCoord.out.bam | 18,641,066 | 0 | 24-76 | 45 |
| 13__-_3Aligned.sortedByCoord.out.bam | 17,605,122 | 0 | 23-76 | 45 |
| 14__-_2_2Aligned.sortedByCoord.out.bam | 26,437,496 | 0 | 23-76 | 45 |
| 14__-_2Aligned.sortedByCoord.out.bam | 25,045,051 | 0 | 23-76 | 45 |
| 15__-_1_2Aligned.sortedByCoord.out.bam | 28,019,239 | 0 | 23-76 | 45 |
| 15__-_1Aligned.sortedByCoord.out.bam | 26,516,612 | 0 | 23-76 | 45 |
| 2_FAD_4_2Aligned.sortedByCoord.out.bam | 24,398,666 | 0 | 24-76 | 46 |
| 2_FAD_4Aligned.sortedByCoord.out.bam | 23,157,372 | 0 | 23-76 | 46 |
| 3_FAD_3_2Aligned.sortedByCoord.out.bam | 24,672,290 | 0 | 22-76 | 45 |
| 3_FAD_3Aligned.sortedByCoord.out.bam | 23,380,344 | 0 | 23-76 | 45 |
| 4_FAD_2_2Aligned.sortedByCoord.out.bam | 23,388,035 | 0 | 23-76 | 45 |
| 4_FAD_2Aligned.sortedByCoord.out.bam | 22,123,347 | 0 | 22-76 | 45 |
| 5_FS_2_2Aligned.sortedByCoord.out.bam | 22,457,813 | 0 | 23-76 | 45 |
| 5_FS_2Aligned.sortedByCoord.out.bam | 21,194,114 | 0 | 23-76 | 45 |
| 6_FAD_1_2Aligned.sortedByCoord.out.bam | 25,406,590 | 0 | 23-76 | 46 |
| 6_FAD_1Aligned.sortedByCoord.out.bam | 24,056,971 | 0 | 23-76 | 46 |
| 7_FS_5_2Aligned.sortedByCoord.out.bam | 27,134,036 | 0 | 23-76 | 45 |
| 7_FS_5Aligned.sortedByCoord.out.bam | 25,628,465 | 0 | 23-76 | 45 |
| 8_FS_4_2Aligned.sortedByCoord.out.bam | 25,057,514 | 0 | 23-76 | 45 |
| 8_FS_4Aligned.sortedByCoord.out.bam | 23,784,746 | 0 | 23-76 | 45 |
| 9_FS_3_2Aligned.sortedByCoord.out.bam | 22,482,650 | 0 | 24-76 | 45 |
| 9_FS_3Aligned.sortedByCoord.out.bam | 21,210,412 | 0 | 23-76 | 45 |
Library Sizes ranged between 17,605,122 and 28,019,239 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Hsapiens Transcriptome
GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Transcriptome
Total overrepresented sequences for each library
## The Overrepresented_sequences module was empty in 1_FAD_5_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 1_FAD_5Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 10_FS_1_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 10_FS_1Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 11__-_5_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 11__-_5Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 12__-_4_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 12__-_4Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 13__-_3_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 13__-_3Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 14__-_2_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 14__-_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 15__-_1_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 15__-_1Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 2_FAD_4_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 2_FAD_4Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 3_FAD_3_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 3_FAD_3Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 4_FAD_2_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 4_FAD_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 5_FS_2_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 5_FS_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 6_FAD_1_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 6_FAD_1Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 7_FS_5_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 7_FS_5Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 8_FS_4_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 8_FS_4Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 9_FS_3_2Aligned.sortedByCoord.out_fastqc.zip
## The Overrepresented_sequences module was empty in 9_FS_3Aligned.sortedByCoord.out_fastqc.zip
## No overrepresented sequences were detected by FastQC
Universal Adapter Content
## The Kmer_Content module was missing from 1_FAD_5_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 1_FAD_5Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 10_FS_1_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 10_FS_1Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 11__-_5_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 11__-_5Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 12__-_4_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 12__-_4Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 13__-_3_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 13__-_3Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 14__-_2_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 14__-_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 15__-_1_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 15__-_1Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 2_FAD_4_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 2_FAD_4Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 3_FAD_3_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 3_FAD_3Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 4_FAD_2_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 4_FAD_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 5_FS_2_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 5_FS_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 6_FAD_1_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 6_FAD_1Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 7_FS_5_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 7_FS_5Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 8_FS_4_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 8_FS_4Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 9_FS_3_2Aligned.sortedByCoord.out_fastqc.zip
## The Kmer_Content module was missing from 9_FS_3Aligned.sortedByCoord.out_fastqc.zip
## No Kmers were detected by FastQC